zymo dna clean (Zymo Research)
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Zymo Dna Clean, supplied by Zymo Research, used in various techniques. Bioz Stars score: 95/100, based on 172 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+clean+concentrator+100/DNA+Clean+%26+Concentrator-100/pmc13056364-243-7-7
Average 95 stars, based on 172 article reviews
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1) Product Images from "DNA O-MAP uncovers the molecular neighborhoods associated with specific genomic loci"
Article Title: DNA O-MAP uncovers the molecular neighborhoods associated with specific genomic loci
Journal: eLife
doi: 10.7554/eLife.102489
Figure Legend Snippet: ( A ) Workflow of DNA O-MAP integrated with sample multiplexing quantitative proteomics. ( B ) Schematic of the three DNA loci examined in the TMT16plex experiment: pericentromeric alpha satellites, telomeres, and mitochondrial genomes. ( C ) Co-localization of DNA fluorescent in situ hybridization (FISH) and the streptavidin staining of the proteins biotinylated by DNA O-MAP targeting the pericentromeric alpha satellites, telomeres, and mitochondrial genomes. Scale bar: 5 µm. ( D ) Principal component analysis of scaled intensities of proteins enriched by the pan-alpha probe, telomere probe, mitochondrial genome oligo pool, and no-primary-probe control. ( E ) Unsupervised hierarchical clustering of scaled intensities of proteins enriched by the pan-alpha probe, telomere probe, mitochondrial genome oligo pool, and no-primary-probe control. ( F ) Log 2 fold change of proteins compared to no-primary-probe control, grouped by HPA subcellular location. Significance calculated based on Welch’s t -test for pairwise comparisons (****p-value <0.0001). Log 2 fold change of proteins compared to mitochondrial probe enriched proteins for the RNA Polymerases ( G ), mtDNA nucleoid packaging proteins ( H ), Shelterin ( I ), and CENP-A nucleosomal complexes ( J ). Significance calculated based on Welch’s t -test for pairwise comparisons (p-value: *<0.05, **<0.01, ***<0.001, ****<0.0001).
Techniques Used: Multiplexing, Quantitative Proteomics, In Situ Hybridization, Staining, Control
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